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The ultimate goal of AI is to build a system that can mimic human intelligence. A. True B. False
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Using a DNA Extraction Protocol Describe two possible negative consequences that could occur if you forget to add Proteinase K at the
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Why do you need a forward and reverse primer in PCR?
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Define Genomics, Proteomics, and Bioinformatics and discuss how they are used for studying gene expression.
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What is the main purpose of flowers?
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What is a cell culture hood?
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How do you know if a cell culture is contaminated?
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What are monkey face orchids?
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What type of microscope is best suited for looking at living cells in tissue culture?
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Are ferns herbaceous plants?
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Why is DMEM used in cell culture?
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What are the main differences between shotgun sequencing and clone based sequencing?
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What is the inheritance pattern for sickle-cell anemia?
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What is biotechnology and what can it be used for?
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What is PBS used for in cell culture?
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Describe one specific example of a genetically modified organism (GMO).
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You order a PCR primer from Eurofins Genomics. They deliver a tube with 35 nmoles of DNA in it. How much TE buffer do you need to turn it into a 100 ?M freezer stock? Which pipettor should you use? To what should it be set?
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What is another name for a flowering plant?
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What is a pedigree chart? What are its uses? How can we make predictions using it?
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What are the advantages and disadvantages of Next Generation DNA Sequencing?
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What is the role of the thermocycler in PCR reaction? a) buffers the reaction to maintain the proper pH. b) It establishes the proper temperatures to regulate the steps of the PCR reaction. c) It heats the PCR tubes to boiling and holds them at 95°C t
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Use the restriction map provided for the pUC19 plasmid to answer the question below. Note that this map shows only a limited number of restriction enzyme sites. The plasmid is 6500 base pairs (bp) around. The box labeled amp^r is the ampicillin-resistance
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List all of the needed components of PCR and what the function is of each.
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The 2x PCR Master has four main components. Two of these are PCR Buffer and MgCl2. What are the other two components and what are their functions?
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Describe the monoclonal antibody production process.
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Why add beta mercaptoethanol to cell culture media?
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How many adapter(s) and primer(s) does illumina platform require to sequence one sample? a) 2 adapters, 1 primers b) 2 adapters, 2 primers c) Only 2 adapters d) 4 adapters, 2 primers.
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What extinct animals have been cloned?
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Conduct a trypan blue exclusion test of cell viability. Make a 1:1 ratio of cells: dye. Count an average of 45 love cells and 5 dead cells per mm square. 1. Calculate cell density 2. Calculate percent viability
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What is DNA activation?
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What Tm value (to 1 decimal place) would you use for the reverse primer when calculating the annealing temperature for the first 5 cycles of PCR? Assume the reverse primer sequence is 5′ CTC GAG AAA GAC AAA TTT GCA TAT ACA G 3′.
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Two short-tailed cats are mated. The litter contains 2 kittens without tails, 5 kittens with short tails, and 1 kitten with a long tail. How do you explain this genetically? Use symbols to support your explanation.
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a. What type of mutation causes sickle cell anemia? What type of mutation causes Huntington’s disease? b. What is the effect of those mutations on the structure of the protein? c. Are these mutations present in the DNA, RNA, or both? d. Are these mutation
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What is in vitro cell culture?
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What are examples of flowering plants?
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Describe the applications of polymerase chain reaction (PCR) and DNA fingerprinting.
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Determine the volume of cell culture needed to plate at a cell density of 22,000 cells/cm^2 in a 24 well plate. The cell culture is at a density of 960,000 cells/mL.
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What are the benefits of biotechnology in agriculture?
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A DNA sequence is 1500 nucleotides long. Answer the following questions: A) How many amino acids does this sequence code for? B) How many mRNA strands does this sequence code for? C) How many proteins does this sequence code for? D) Would it be more harmf
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What is polymerase chain reaction (PCR) and why it is important in DNA analysis?
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