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Electromagnetic radiation
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How do you calculate the nucleic acid concentration in g/mL from the following information below? DNA: A reading at 260 nm = 0.307 RNA: A reading at 260 nm = 0.307 DNA: A reading at 260 nm from a 1:100 dilution = 0.086 DNA: A reading at 260 nm from
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Electromagnetic radiation
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What is the bright line spectrum?
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Electromagnetic radiation
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What are the different sources of light? Describe how each of them is created.
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Electromagnetic radiation
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Rank labels from highest wavelength to lowest. (a) the gamma rays produced by a radioactive nuclide used in medical imaging (b) radiation from an FM radio station at 93.1 MHz on the dial (c) a radio signal from an AM radio station at 680 kHz on the dial
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What is the function of a monochromator?
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Electromagnetic radiation
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Listener supported King FM is found at 98.1 Hz on your FM dial. What is the wavelength of these radio waves?
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Electromagnetic radiation
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You are given the task of identifying an unknown substance found at a crime scene. Which method would be better for you to implement to identify elements present in the substance, using a series of flame tests or analyzing the emission spectra?
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Electromagnetic radiation
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In an old house, the heating system uses radiators, which are hollow metal devices through which hot water or steam circulates. In one room the radiator has a dark color (emissivity = 0.804). It has a
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Electromagnetic radiation
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The absorbance of the solution in 1 cm cuvette is 0.15. If you use 0.5 cm, what would be the absorbance of the same solution?
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Electromagnetic radiation
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A 0.10 M solution of compound “B” is measured in a 1.00 cm path length cuvette at a wavelength of 500 nm. The percentage of light transmitted through the cell is 38%. a. What is the absorbance of this sample (A) at 500 nm? b. Assuming Beer’s Law holds, wh
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Electromagnetic radiation
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Explain why the ultraviolet spectrum of benzene vapor contains many sharp bands, whereas the same spectrum of benzene solution in alcohol lacks fine detail and consists only of broad peaks.
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Electromagnetic radiation
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Given that the path length of the cuvette is 1 cm, what is the extinction coefficient of the 0.020 mM Yellow 5 dye at lambda max? Given the data in the table below, what is the concentration of the di
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Electromagnetic radiation
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A Plano-Convex lens has a focal length of 30 cm. Find the radius of the convex surface if its index of refraction is 1.55.
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Electromagnetic radiation
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Lasers are used to burn and read CDs. Explain why a laser that emits blue light would be capable of burning and reading more information than one that emits infrared.
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Electromagnetic radiation
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Nitrate levels in well water can be determined spectrophotometrically through a procedure that measures the intensity of absorbed light at 410 nm after the sample has been properly treated with phenol disulfonic acid. An unknown sample of 25.0 mL of well
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Electromagnetic radiation
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How does a nanodrop spectrophotometer work?
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Electromagnetic radiation
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How does a spectrophotometer measure DNA concentration?
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Electromagnetic radiation
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What is the difference between nanodrop and UV spectrophotometer? And which one is preferable for measuring the concentration of extracted DNA/RNA?
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Electromagnetic radiation
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Suppose an AM talk radio station broadcasts radio waves with a frequency of 740 kHz. Calculate the wavelength of these radio waves.
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Electromagnetic radiation
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Is communication faster than the speed of light?
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Electromagnetic radiation
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If you cannot see through a window, you might say it is: a. Refracted b. Translucent c. Opaque d. Opine e. Transparent
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Electromagnetic radiation
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Consider Beer’s Law: A = kC a. If concentration (C) is given in units of mg Fe/mL solution, what are the units of the constant, k? Remember, A is unitless. b. The percent transmittance of a solution is measured to be 72.1%. Convert this to absorbance. W
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What is the range of wavelengths for ultraviolet (UV) light?
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Electromagnetic radiation
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A He-Ne laser cavity has a cylindrical geometry with length 30 cm and diameter 0.5 cm. The laser transition is at 633 nm, with a frequency width of 10 nm. (a) Determine the number of modes in the lase
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Optical properties of bacterio-chlorophyll e (BChl e): epsilon = 142300 L mol^-1 cm^-1 in ethanol @ 469 nm (blue light) l = 1 mm, Molar Weight = 835.1 g/mol, m(BChl e) = 0.1 g, V = 1 L a) What’s the
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Electromagnetic radiation
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An analyst decides to determine the extent of a milk fermentation by measuring the amount of lactic acid in the milk using an enzyme assay. Lactic acid is measured by adding 0.05 mL of fermented milk to a cuvette along with the enzyme, lactate dehydrogena
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Determine the appropriate LED color and wavelength (in nm) that should be used to measure the absorbance of red, blue, and yellow dye solutions.
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Electromagnetic radiation
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What is the first color of the rainbow?
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Electromagnetic radiation
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What are the differences between polarized and unpolarized light?
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Electromagnetic radiation
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a.) Sirius B is a white star that has a surface temperature (in kelvins) that is four times that of our sun. Sirius B radiates only 0.040 times the power radiated by the sun. Our sun has a radius of 6
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Why does a neon discharge lamp produce more distinct spectral lines than a hydrogen lamp?
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Electromagnetic radiation
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What would be observed in an atomic emission spectrum if electrons could exist anywhere in an atom?
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Electromagnetic radiation
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The wavelength of a particular color of red light is 688 nm. The frequency of this color is ________ sec^{-1}. (1 nm = 10^{-9} m)
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Electromagnetic radiation
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Radio waves with wavelengths less than a meter are called
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Electromagnetic radiation
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The concentration of a protein can, as a rule of thumb, be calculated by the absorbance at 280 nm (caused mostly by tryptophan and tyrosine). At a concentration of 1 mg/mL and a cuvette dimension of 1 cm, the extinction at 280 nm is 1.
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Electromagnetic radiation
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Does glass reflect heat?
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How are the colors in a spectrum from an atom different from those in a rainbow?
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Electromagnetic radiation
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The bright-line spectra produced by four elements are represented in the diagram below. Given the bright-line spectrum of a mixture formed from two of these
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Electromagnetic radiation
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What is the frequency of ultraviolet light with a wavelength of 200 nm?
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Electromagnetic radiation
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A lab analysis prepared the set of ethanol standards and a blank shown below. The absorbance values were read on a UV-visible spectrophotometer at a wavelength of 270 nm. \ A. Plot the standard curve for corrected absorbance versus concentration (indepen
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