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Biochemistry
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The enzyme, RNA polymerase, is needed to transcribe DNA into mRNA, which is then translated into proteins (such as the polymerase). But the genetic code for this enzyme protein is within the DNA. Based on your reading, please explain how you think a polym
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Biochemistry
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Why did Beadle and Tatum have to revise the one gene-one enzyme hypothesis to the one gene-one primary function hypothesis?
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Biochemistry
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Gene expression consists of two major processes: transcription and translation. Describe both of these processes from binding of the DNA by transcription factors to release of ribosomes following a stop codon. Unlike in eukaryotes, these processes can occ
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Biochemistry
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Explain what a GFP-Fusion Protein is, and what it is used for in cell biology research.
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Biochemistry
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What is the amino acid sequence for this DNA sequence: T A C A C C T T G G C G A C G A C T?
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Biochemistry
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What does it mean to have amplification in a cell signaling pathway?
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Biochemistry
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Why are cells alive but macromolecules are not?
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Biochemistry
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True or False: To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used.
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Biochemistry
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Transcribe the following DNA strand into mRNA, and translate that strand into a polypeptide chain, identifying the codons and amino acid sequence. a. DNA: A T A C G A A A T C G C G A T C G C G G C G A T T C G G mRNA: Codons: Anticodons: Amino Acids:
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Biochemistry
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What volume range is a P20 (micropipette) useful for?
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Biochemistry
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What information would you get from in situ hybridization that you would not get from a northern blot when used to analyze the same mRNA?
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Biochemistry
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If a DNA strand is represented by the bases A-C-G-T, what type of molecule will result from transcription and what are its bases?
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Biochemistry
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Which process converts the nucleotide language of mRNA into the amino acid language of a protein? A. transcription B. DNA replication C. meiosis D. translation
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Biochemistry
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which micropipettor would be appropriate to measure 250 microliters? P20, P200, or P1000?
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Biochemistry
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What is the expressed sequence of DNA that codes for proteins?
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Biochemistry
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When does a cell regulate gene expression?
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Biochemistry
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Micropipettes are measured in microliters (1000 l = 1 ml). What would be the digital readout for micropipettor for a reading of 070 in microliters?
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Biochemistry
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In Central Dogma: DNA is [{Blank}] into RNA, and RNA is [{Blank}] into proteins. 1) Translated; transcribed 2) Replicated; translated 3) Transcribed; translated 4) Replicated; transcribed
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Biochemistry
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If 30% of the calories in a 650-calorie meal come from carbohydrates, how many grams of carbohydrates are in that meal?
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Biochemistry
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What advantages does fluorescent labeling offer over radioactive methods of labeling DNA?
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Biochemistry
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Coordinate regulation of gene expression is important both in bacteria and eukaryotes. Operons are used to coordinate gene expression in bacteria but not eukaryotes. Explain why the operons strategy does not work in eukaryotes.
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Biochemistry
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Explain how a cDNA library is constructed and how it is different from genomic libraries constructed using plasmids or phages.
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Biochemistry
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What does the term ”range” mean in regard to a micropipettor?
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Biochemistry
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Ariel received a lineage of “knockout mice” from a collaborator. What method would not be useful to confirm that the mice really do not express the targeted gene in all tissues of the body? A. Northern blot B. Neomycin sensitivity assay C. Western blot D.
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Biochemistry
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What technique is used to determine the 3D structure of a protein?
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Biochemistry
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Humans started using elemental copper about 6000 years ago and started using elemental tin about 3800 years ago. Using the heat of formation values for copper(II) oxide and tin (IV) oxide, explain why humans were able to use elemental copper before they w
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