Tech and Engineering

admin

29.07.2022 - 18:11

What is the purpose of adding tracking dye to the DNA samples?

Question:

What is the purpose of adding tracking dye to the DNA samples?

Answers (1)

Natasha April 7, 2023 в 22:32

The purpose of adding tracking dye to the DNA samples is to visualize the DNA migration during gel electrophoresis. Tracking dyes are typically added to DNA samples before loading them onto an agarose gel. The tracking dye contains a colored molecule that moves with the DNA as it migrates through the gel.
During gel electrophoresis, DNA molecules are separated based on their size and charge. The negatively charged DNA molecules move towards the positively charged electrode and migrate through the gel matrix. The tracking dye helps to visualize the DNA migration as it moves through the gel, allowing researchers to determine the size and amount of DNA in the sample.
The choice of tracking dye may vary depending on the application, but commonly used tracking dyes include bromophenol blue, xylene cyanol FF, and orange G. These dyes do not interfere with DNA migration and have distinct colors that are easy to distinguish, allowing researchers to quickly identify the location of DNA bands on the gel.

Do you know the answer?

Leave a comment Cancel Response

Not sure about the answer?

Find the right answer to the question What is the purpose of adding tracking dye to the DNA samples? by subject Tech and Engineering, and if there is no answer or no one has given the right answer, then use the search and try to find the answer among similar questions.

Search for other answers

New questions in the category: Tech and Engineering

Is business intelligence the same as intelligent systems?

An average car consumes about 5 L of gasoline a day, and the capacity of the fuel tank of a car is about 50 L. Therefore, a car needs to be refueled once every 10 days. Also, the density of gasoline ranges from 0.68 to 0.78 kg/L, and its lower heating val

A circular bar of magnesium alloy is 32 in long. Answer the following questions using the strain graph below. (a) What is the proportional limit of the bar? (b) What is the modulus of Elasticity of

A 22-kilo ohm resistor having a tolerance of 10% has failed. You have on hand five resistors, valued at 120, 220, 47, 33 and 120-kilo ohm, respectively. What combination of these would you choose to re

How much current flows in a 1000-ohm resistor when 1.5 volts are impressed across it?

Below are blood glucose graphs from two individuals who may be lactose intolerant. Which of the graphs belongs to a patient with lactose intolerance?

A person wants to launch a water balloon and hit the top of a tower, 50.0 meters away. The water balloon launcher is angled at 25 degrees. The water balloon leaves the launcher at 68 m/s. If the ballo

A 221 V battery, an inductor, and a 33 ohms resistor are connected in series, as shown in the diagram below. A two way switch makes it possible to include or exclude the battery. The switch that had been in position 1 long enough for the circuit to reach

A 10 micro F capacitor has been charged to a potential difference of 12 V. A 100 k ohm resistor is connected in series with the capacitor. What are the potential difference across the resistor, curren

What is the total resistance (RT) in the circuit? a. R1 = 7 ohms b. R2 = 10 ohms c. R3 = 6 ohms d. R4 = 4 ohms

Classify the microorganisms based on their (i) respiration (ii) carbon source and (iii) energy source.

A rechargeable flashlight battery is capable of delivering 90 mA for about 12 hours. How much charge can it release at that rate? If its terminal voltage is 1.5 V, how much energy can the battery deliver?

A 4.5V battery produces a current of 0.90A in a piece of copper wire. What is the resistance of the copper wire?

A 100 N force is applied to the side of a crate resting on a level floor. The crate has a mass of 50 kg. If the coefficient of static friction is s = .25, is the 100 N force enough to make the crate

The circuit breaker at a power seat motor continuously trips: Technician A says that this could be caused by a seat track problem that is causing mechanical resistance. Technician B says that this could be caused by corrosion at the motor, which is causin

Q. Large spool of copper wire is placed on 30 degree inclined plane. The loose end of the wire comes off of the top edge of the spool and is attached to a rigid pole at the top edge of the incline. Th

Discuss the zero-sum and the non-zero-sum games in international relations.

Each of the 884 floodlights at Yankee Stadium uses electrical energy at the rate of 1650 W. How much does it cost to run these lights during a 5-h night game, if electricity costs 21 c/kW.h?

What are the types of cybercrime perpetrators?

The restriction enzyme HaeIII recognizes and cuts the DNA sequence 5′-GGCC-3′ 3′-CCGG-5′ A. How many DNA fragments would be expected to be produced upon digestion of the plasmid pUC18DNA with HaeIII (plasmid pUC 18 contains 2686 base-pairs of DNA). B. W

Authorization

Password generation