What is the difference between SDS buffer solution and BE buffer solution?

SDS buffer solution is used for polyacrylamide gel electrophoresis (PAGE) to separate proteins based on their molecular weight. It contains the detergent sodium dodecyl sulfate (SDS) which denatures proteins and makes them negatively charged. This leads to uniform charge-to-mass ratio for all proteins and allows them to be separated solely based on their size. On the other hand, BE buffer solution is used for DNA electrophoresis. It contains boric acid and EDTA which are important for maintaining the stability of DNA and minimizing the effects of the electric field on DNA. It provides a suitable environment for the DNA to migrate through the gel matrix and allows for separation based on size and charge. Therefore, the main difference between SDS buffer solution and BE buffer solution is their composition and purpose of use. SDS buffer is used for protein analysis while BE buffer is used for DNA analysis.