I have a new column for HPLC (high-performance liquid chromatography) analysis. Is it advisable to condition the new column with the mobile phase overnight at a slow flow rate of 0.5 mL/min?

Yes, it is advisable to condition the new column with the mobile phase overnight at a slow flow rate of 0.5 mL/min. This helps to remove any impurities or contamination from the column, ensuring accurate and consistent results in subsequent HPLC analyses. The slow flow rate allows for thorough conditioning without damaging the column, and overnight conditioning ensures that the column is fully equilibrated before use.