20.03.2023 - 09:04

Define the following terms: A. Chromatography B. Mobile phase C. Stationary phase E. Cation F. Cellulose G. Charge density H. retention factor

Question:

Define the following terms:

A. Chromatography

B. Mobile phase

C. Stationary phase

E. Cation

F. Cellulose

G. Charge density

H. retention factor

Answers (1)
  • Falcon
    April 6, 2023 в 15:27

    A. Chromatography is a technique used to separate and identify different components in a mixture. It involves passing the mixture through a stationary phase, which can be a solid or a liquid, and a mobile phase, which moves through the stationary phase and carries the components of the mixture with it. The components in the mixture will move at different rates through the stationary phase, depending on their physical and chemical properties.

    B. The mobile phase is the solvent or gas that carries the mixture through the stationary phase in chromatography. It can be a liquid, such as water or ethanol, or a gas, such as helium or nitrogen. The choice of mobile phase depends on the properties of the mixture and the stationary phase.

    C. The stationary phase is the solid or liquid matrix in chromatography that does not move during the separation process. It can be a solid material, such as silica gel or alumina, or a liquid material, such as water or an organic solvent. The choice of stationary phase depends on the properties of the mixture and the mobile phase.

    E. A cation is an ion with a positive charge. It is formed when an atom loses one or more electrons. Cations are attracted to negatively charged ions or molecules, such as anions or polar molecules.

    F. Cellulose is a polysaccharide that is the main component of plant cell walls. It is made up of glucose monomers that are linked together in long chains. Cellulose is a common stationary phase used in chromatography because of its ability to interact with a wide range of molecules.

    G. Charge density refers to the amount of electric charge per unit volume or area. It is a measure of the concentration of charged particles in a given space. In chromatography, charge density can affect the interaction between the stationary phase and the components of the mixture.

    H. The retention factor (Rf) is a measure of the distance a component travels through the stationary phase relative to the distance traveled by the mobile phase. It is calculated as the distance traveled by the component divided by the distance traveled by the mobile phase. The Rf value can be used to identify the components of a mixture based on their behavior in chromatography.

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