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08.07.2022 - 13:18

A student fails to insert the cuvette into the colorimeter or spectrophotometer correctly. What effect will this have on the measured absorbance?

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A student fails to insert the cuvette into the colorimeter or spectrophotometer correctly. What effect will this have on the measured absorbance?

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Bettie April 18, 2023 в 07:27

If the cuvette is not inserted correctly, it may not be in the path of the light beam, which will result in an incorrect reading. This will cause the measured absorbance to be lower than the actual absorbance because some of the light will not pass through the sample solution. Alternatively, if the cuvette is inserted too deep, it may block the light path completely, resulting in a reading of zero absorbance. Therefore, it is important to ensure that the cuvette is inserted correctly to obtain accurate absorbance measurements.

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